![]() To evaluate the accuracy of the BioPlex HSV assay, 505 prospective serum specimens were tested by enzyme immunoassay (EIA) (HerpeSelect, Focus Diagnostics) and the BioPlex HSV-1/2 IgG assay. Repeat testing, or testing by a different method, may be indicated in some settings (eg, patients with low likelihood of HSV infection). The predictive value of positive or negative results depends on the prevalence of disease and the pretest likelihood of HSV-1 and HSV-2.įalse-positive results may occur. HSV serology cannot distinguish genital from nongenital infections. The presence of IgG-class antibodies to either HSV type 1 or 2 does not differentiate between remote infection or acute disease. In cases of suspected early disease, a repeat serum specimen should be collected 14 to 21 days later and submitted for testing. Serum specimens collected too early in the course of infection may not have detectable levels of HSV IgG. For patients presenting with presumed acute infection with HSV, a clinical specimen (eg, oral, dermal, or genital lesion) should be sampled and submitted for detection of HSV types 1 and 2 by polymerase chain reaction. In addition, the results of HSV type-specific IgG testing are sometimes used during pregnancy to identify risks of congenital HSV disease and allow for focused counseling prior to delivery.ĭetection of IgG-class antibodies to herpes simplex virus (HSV) should not be used routinely as the primary means of diagnosing HSV infection. There are several circumstances where it may be important to distinguish between infection caused by HSV types 1 and 2 (eg, risk of reactivation). However, in instances of subclinical or unrecognized HSV infection, serologic testing for IgG-class antibodies to type-specific HSV glycoprotein G may be useful. The diagnosis of HSV infections is routinely made based on clinical findings and supported by laboratory testing, primarily using polymerase chain reaction to detect viral DNA. HSV type 1 is closely associated with orolabial infection, although genital infection with this virus can be common in certain populations. HSV type 2 primarily causes urogenital infections and is found almost exclusively in adults. Infections with HSV types 1 and 2 can differ significantly in their clinical manifestations and severity. Clinical conditions associated with HSV infection include gingivostomatitis, keratitis, encephalitis, vesicular skin eruptions, aseptic meningitis, neonatal herpes, genital tract infections, and disseminated primary infection. Herpes simplex virus (HSV) types 1 and 2 are members of the Herpesviridae family and produce infections that range from mild stomatitis to disseminated and fatal disease. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |